Publications

2023

Structure of Staphylococcus aureus ClpP Bound to the Covalent Active Site Inhibitor Cystargolide A
Astrid Illigmann*, Marie-Theres Vielberg*, Markus Lakemeyer*, Felix Wolf, Taulant Dema, Patrik Stange, Wolfgang Kuttenlochner, Elisa Liebhart, Andreas Kulik, Nicole Staudt, Imran Malik, Stephanie Grond, Stephan A. Sieber, Leonard Kaysser, Michael Groll, Heike Brötz-Oesterhelt, Angew .Chem .Int .Ed

Chemoproteomic identification of a dipeptidyl peptidase 4 (DPP4) homolog in Bacteroides thetaiotaomicron important for envelope integrity and fitness 
L. J. Keller, T. H. Nguyen, L. Liu, M. Lakemeyer, D.J. Gelsinger, R. Chanin, N. Ngo, K. M. Lum, F. Faucher, P. Ipock, M. J. Niphakis, A. S. Bhatt, A. J. O’Donoghue, K. C. Huang, M. Bogyo, Nat. Chem. Bio., 19,1469-1479.

2022

Antibiotic acyldepsipeptides stimulate the Streptomyces ClpP-ATPase/ClpP complex for accelerated proteolysis

L. Reinhard, D. Thomy, M. Lakemeyer, J. Ortega, S. Sieber, P. Sass, H. Brötz-Oesterhelt, mBio, 13, e01413-22

Uncovering an overlooked consequence of phosphorylation: change in cysteine reactivity
M. Lakemeyer, M. Bogyo, Nat. Methods, 19, 281-283.

Integration of bioinformatic and chemoproteomic tools for the study of enzyme conservation in closely related bacterial species

L. J. Keller, M. Lakemeyer, M. Bogyo, Methods in Enzymol., 664, 1-22.

2021

Toxoplasma gondii serine hydrolases regulate parasite lipid mobilization during growth and replication within the host
O. Onguka, B. M. Babin, M. Lakemeyer, I. T. Foe, N. Amara, S. M. Terrell, K. Lum, P. Cieplak, J. Z. Long, M. Bogyo, Cell Chem. Biol., 28, 1501-1513

2020

Activity-based protein profiling in bacteria: Applications for identification of therapeutic targets and characterization of microbial communities
L. J. Keller, B. M. Babin, M. Lakemeyer, M. Bogyo, Curr. Opin. Chem. Biol., 54, 45–53.

2019

Tailored peptide phenyl esters block ClpXP proteolysis by an unusual breakdown into a heptamer-hexamer assembly
M. Lakemeyer, E. Bertosin, F. Möller, D. Balogh, R. Strasser, H. Dietz, S.A. Sieber, Angew. Chem. Int. Ed. Engl., 58, 7127–7132.

2018

Thinking outside the box: novel antibacterials to tackle the resistance crisis
M. Lakemeyer, W. Zhao, F. A. Mandl, P. Hammann, S.A. Sieber, Angew. Chem. Int. Ed. Engl., 57, 14440–14475.

 

Design and synthesis of tailored human caseinolytic protease P inhibitors
T. F. Gronauer, M. M. Mandl, M. Lakemeyer, M. W. Hackl, M. Meßner, V. S. Korotkov, J. Pachmayr, S. A. Sieber, Chem. Commun. (Camb.), 54, 9833–9836.

2017

Quantitative map of beta-lactone induced virulence regulation
J. Krysiak, M. Stahl, J. Vomacka., C. Fetzer, M. Lakemeyer, A. Fux, S.A. Sieber,  J. Proteome. Res., 16, 1180–1192.

2015

Reversible inhibitors arrest ClpP in a defined conformational state that can be revoked by ClpX association
A. Pahl*, M. Lakemeyer*, M.-T. Vielberg*, M.W. Hackl, J. Vomacka, V.S. Korotkov, M. Stein, C. Fetzer, K. Lorenz-Baath, K. Richter, H. Waldmann, M. Groll, S.A. Sieber, Angew. Chem. Int. Ed. Engl., 54, 15892–15896.


Phenyl esters are potent inhibitors of caseinolytic protease P and reveal a stereogenic switch for deoligomerization
M.W. Hackl*, M. Lakemeyer*, M. Dahmen, M. Glaser, A. Pahl, K. Lorenz-Baath, T. Menzel, S. Sievers, T. Böttcher, I. Antes, H. Waldmann, S.A. Sieber, J. Am. Chem. Soc., 137, 8475–8483.

* These authors contributed equally to the work.